A Direct Plaque Assay for Hog Cholera Virus

Reverse transcriptase-PCR assay for detection of hog cholera virus.

A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.

Detection and segmentation of virus plaque using HOG and SVM: toward automatic plaque assay.

Plaque assaying, measurement of the number, diameter, and area of plaques in a Petri dish image, is a standard procedure gauging the concentration of phage in biology. This paper presented a novel and effective method for implementing automatic plaque assaying. The method was mainly comprised of the following steps: In the training stage, after pre-processing the images for noise suppression, a...

Modified Live Virus Hog Cholera Vaccine--M.L.V

The structural components of hog cholera virus.

Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S]methionine and [3H]uridine. At least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was det...

Hog cholera virus: art and facts.

The aim of this review is to summarize the informations accumulated during the two last decades on hog cholera virus, a member of the pestivirus genus. Different aspects concerning the virus-host cell interactions, and the structural and antigenic properties of the virion itself are successively analyzed. Despite numerous works, many basic informations are still lacking, which can be explained ...